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Wednesday, April 22, 2020 | History

2 edition of Solvent extraction of dextran from fermentation products found in the catalog.

Solvent extraction of dextran from fermentation products

S. J. Sibson

Solvent extraction of dextran from fermentation products

  • 84 Want to read
  • 26 Currently reading

Published by UMIST in Manchester .
Written in English


Edition Notes

StatementS.J. Sibson ; supervised by G.A. Davies.
ContributionsDavies, G. A., Supervisor., Chemical Engineering.
ID Numbers
Open LibraryOL21846713M

Efficiency of Liquid-liquid Extraction. Use In Classroom Create Test. When there is a reasonable density difference between the feed and solvent streams, extraction can be accomplished using a vertical column, although in other cases a series of mixing and settling tanks may be used. ranging from citric acid extraction from fermentation. Extraction of citric acid from a broth. A clarified broth of 1, kg/h from the fermentation of sucrose using Aspergillus niger consists of wt % citric .


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Solvent extraction of dextran from fermentation products by S. J. Sibson Download PDF EPUB FB2

Dextran extraction by solvent precipitation The final extract (MF-Retentate) containing the dextran was precipitated using ethanol (, v/v) and then stored at 4 ± 1 °C for 72 h.

The dextran was dried at 50 ± 1 °C during 24 h and later pulverized with a milling : Elsa Díaz-Montes, Jorge Yáñez-Fernández, Roberto Castro-Muñoz. Direct solvent extraction: This process involves the use of a nonpolar solvent, usually hexane, to dissolve the oil without removing proteins and other non-oil soluble compounds.

Solvent extraction yields about % more oil than does the screw press method, and less oil remains in the meal. In liquid extraction of fermentation products, components dissolved in liquid are recovered by transfer into an appropriate solvent.

Extraction of penicillin from aqueous broth using solvents such as butyl acetate, amyl acetate, or methyl isobutyl ketone, and isolation of erythromycin using pentyl or amyl acetate.

The extraction with organic solvents is likely the most used methodology to recover the metabolites from the fermentation broths; which has been criticized according to the negative effect of. Fermentation and Biochemical Engineering Handbook Extraction requires that the solvent and feed phases be of different densities, Even though extraction may successfilly remove the solute from the feed, a fbrther separation is required in order to recover the solute from the solvent, and to make the solvent suitable for reuse in the Size: 1MB.

The dextran produced by Leuconostoc mesenteroides SF3 has been fully characterized. • An integrated MDF-MF system has been, for the first time, used for dextran recovery. • A comparison between solvent extraction and MDF-MF has been performed.

• The use of the membrane technology promotes the reduction of ethanol : Elsa Díaz-Montes, Jorge Yáñez-Fernández, Roberto Castro-Muñoz. Extraction is the removal of a soluble constituent from one liquid into -liquid extraction is a unit operation frequently employed for recovery and purification of a desired.

In a four-stage countercurrent extraction with the volume ratio of the extraction solvent to the aqueous phase beingmore than 99% 1,3-propanediol acetal (2-propyl-1,3-dioxane) and 2,3. amount of the dextran and of the by-products produced from sucrose by cultures of Leuconostoc mesenteroides.

A factor of outstanding importance is the strain of the organism (5), which appears to determine whether the dextran is water-soluble or water-insoluble (3, 6). The structural basis. dextran in these products will have a nutritional significance for consumers.

Information from previous human exposure to dextran Dextran has a long history of clinical use as a blood extender. A 6% solution of a dextran fraction was approved for clinical use in Sweden in and shortly thereafter in the UKFile Size: 50KB. Fermentative Production of Dextran using Food Industry Wastes a comparison between dextrans extracted with solvent extraction (i.e., ethanol) and integrated MF system is presented.

Besides solvent extraction, novel separation techniques with liquid membrane, microemulsion and reversed micelles are also presented. This book will introduce the biochemical engineer and process engineer to the recovery of products from complex cultivation broths by modern techniques of solvent extraction and help them with process by: Solvent extraction is employed very widely in both fundamental research and technology because of the remarkable features of this simple but very effective technique for the separation of different materials.

The International Solvent Extraction Conference was a forum for the presentation of papers on up-to-date research in this field. A complete and up-to-date presentation of the fundamental theoretical principles and many applications of solvent extraction, this enhanced Solvent Extraction Principles and Practice, Second Edition includes new coverage of the recent developments in solvent extraction processes, the use of solvent extraction in analytical applications and waste recovery, and computational chemistry methods Reviews: 1.

S.J Sibson has written: 'Solvent extraction of dextran from fermentation products' Asked in Chemistry, History and Origins of Foods, Biochemistry Is dextrin the same as dextran. 1 Extraction of Natural Products from Plants – An Introduction Hans - J ö rg Bart Introduction The history of the extraction of natural products dates back to Mesopotamian and Egyptian times, where production of perfumes or pharmaceutically - active oils and waxes was a major business.

Herein, a comparison between dextrans extracted with solvent extraction (i.e., ethanol) and integrated MF system is presented. The results revealed that the membrane process modified some of the physicochemical (e.g., hygroscopicity, solubility, water absorption capacity, and porosity) and morphological characteristics of the dextran.

Ionic liquids are already applied in green extraction of natural products for their solvent power, high chemical and thermal stability, and as a non-flammable and non-VOC solvent. For example, in the case of extraction of artemisinin, by using ionic liquids, pure compound is obtained after simple precipitation [ 4 ].Cited by: A complete reference for fermentation engineers engaged in commercial chemical and pharmaceutical production, Fermentation and Biochemical Engineering Handbook emphasizes the operation, development and design of manufacturing processes that use fermentation, separation and purification techniques.

Contributing authors from companies such as Merck, Eli Lilly, Amgen and Bristol-Myers. Dextran is a complicating contaminant in the refining of sugar because it elevates the viscosity of sucrose solutions and fouls plumbing.

Dextran is now produced from sucrose by certain lactic acid bacteria of the family lactobacillus. Species include Leuconostoc mesenteroides and Streptococcus mutans. The structure of dextran produced depends not only on the family and species of the CAS Number:   Introduction.

Dextran is an extracellular bacterial polymer of D-glucopyranose with predominantly α- (1→6) linkage in the main chain and a variable amount of α-(1→2), α-(1→3), α-(1→4) branched linkages Other workers have also reported formation of dextran from different strains of bacteria that were primarily Leuconostoc specificity of the synthesized linkages in Cited by: The liquid-liquid extraction of proteins directly from fermentation media or cell homogenates is an attractive primary purification process and can be used both to remove cell debris and to.

Applications of solvent extraction 1. Analytical Chemistry Applications of solvent extraction Names of participants Ayesha Saleem, Namra Babar, Amina Ashraf, Ayesha Bibi, Kainat Sajjad, Wardah Riaz Roll numbers. 21, 51,29,37,09 Semester 5th (A) Instructor Dr. Maria 2. Theeffects of four extracting solvents [absolute ethanol, absolute methanol, aqueous ethanol (ethanol: water, v/v) and aqueous methanol (methanol: water, v/v)] and two extraction techniques (shaking and reflux) on the antioxidant activity of extracts of barks of Azadirachta indica, Acacia nilotica, Eugenia jambolana, Terminalia arjuna, leaves and roots of Moringa oleifera, fruit of Cited by: ¾The efficiency of extraction in a maceration process can be improved by arranging for the solvent to be continuously circulated through the drug.

as indicated in the Fig., given below. ¾Solvent is pumped from the bottom of the vessel to the inlet where it is distributed File Size: KB. This book is intended to be a first step towards a future cooperation in a new extraction of natural products, built to improve both fundamental and green parameters of the techniques and to increase the amount of extracts obtained from renewable resources with a minimum consumption of energy and solvents, and the maximum safety for operators.

The evaluation of the potential for separation of aqueous ethanol mixtures by liquid−liquid solvent extraction has been complicated by inconsistent results and differing experimental methodologies among researchers developing distribution coefficient data. A screening method for measuring equilibrium distribution coefficients for solvent extraction of ethanol from dilute aqueous solutions is Cited by: Alternatively, extraction and stripping (back extraction) can be physically separated into two hollow-fiber (HF) membrane contactors with solvent continuously circulating through them (see Figure 5).

This process requires only a small amount of solvent that is continuously regenerated and used to extract carboxylic acid from the fermentation broth with a good long-term stability [ 90 ]. Extraction can successfully be used for in-situ alcohol recovery in butanol fermentations to increase the substrate conversion.

An advantage of extraction over other recovery methods may be the high capacity of the solvent and the high selectivity of the alcohol/water separation. Extraction, however, is a comprehensive operation, and the design of an extraction apparatus can be by: Liquid–liquid extraction (LLE), also known as solvent extraction and partitioning, is a method to separate compounds or metal complexes, based on their relative solubilities in two different immiscible liquids, usually water (polar) and an organic solvent (non-polar).

There is a net transfer of one or more species from one liquid into another liquid phase, generally from aqueous to organic. Extraction techniques of Medicinal plants Extraction, as the term is used pharmaceutically, involves the separation of medicinally active portions of plant or animal tissues from the inactive or inert components by using selective solvents in standard extraction procedures.

The products so File Size: KB. USA1 US10/, USA USA1 US A1 US A1 US A1 US A US A US A US A1 US A1 US A1 Authority US United States Prior art keywords ether glycol water method phase Prior art date Legal status (The legal status is an Cited by: 8.

This article throws light upon the five stages in downstream processing. The five stages are: (1) Solid-Liquid Separation (2) Release of Intracellular Products (3) Concentration (4) Purification by Chromatography and (5) Formulation. In Fig.an outline of the major steps in downstream processing is given.

Extraction is the first step to separate the desired natural products from the raw materials. Extraction methods include solvent extraction, distillation method, pressing and sublimation according to the extraction principle.

Solvent extraction is the most widely used method. The extraction of natural products progresses through the following Cited by: USA Home > Product Directory > Biochemicals and Reagents > Carbohydrates > Dextrans > Dextran Standards for Gel Permeation Chromatography (GPC) Life Science Home Life Science Products.

The PEG-dextran ATPS system was found to be suitable for the growth of A. pullulans in top phase and partitioning of pullulan to the other phase. The yield of the extractive fermentation process was found to be g/L, slightly higher than organic solvent precipitation process ( g/L) and lesser than ATPS process ( g/L).Cited by: 2.

The basic problems of applying solvent extraction to ethanol fermentation were investigated. The selection of solvents was based on the selectivity ratio, which was expressed as the ratio of the ethanol distribution coefficient to the water distribution coefficient.

Solvents with high selectivity ratios of more than 50 were found mainly among the alcohols and by: Organic solutions of amines are increasingly being used to separate organic acids from aqueous solutions by reactive extraction.

This article deals with experimental investigations of the extraction of citric acid using mixtures of trioctyl methylammonium chloride + cyclohexane, 2,2,4-trimethyl pentane, 1-butanol, toluene, methyl isobutyl ketone, and ethyl acetate.

All measurements were Cited by: 5. Liquid extraction is used to isolate many pharmaceutical products. In liquid extraction of fermentation products, components dissolved in liquid are recovered by transfer into an appropriate solvent.

In the isolation of penicillin, the drug is extracted from its aqueous broth using butyl acetate. 9 2. Structure, physical-chemical properties and reactivity Structure The dextran elaborated by Leuconostoc mesenteroides NRRL B(F) consists of an a(1 6)-linked glucan with side chains attached to the 3-positions of the backbone glucose Size: KB.

Liquid-liquid extraction of biomolecules in downstream processing - a review paper. a mixture of dextran and polyethylene glycol dissolved in water is turbid above certain polymer concentrations, and the two phases are in equilibrium. Journal of Fermentation and Bioengineering, 73, N. The extraction of polyphenols from plants is the first step in utilization of these phytochemicals in nutritional supplements, food ingredients, cosmetic and pharmaceutical products.

The phenolic compounds can be extracted from dried or fresh plant materials. The most common method to obtain extracts from plants is solvent : Ioana Asofiei, Ioan Calinescu, Adrian Trifan, Adina Ionuta Gavrila.Botanical Extraction is placing a solid (plant) in a solvent in order to remove soluble (dissolvable) components, similar to making coffee or tea.

Extraction techniques are used to separate the components and remove them from the plant matrix. Various methods can divide plant material into parts, or extracts, that contain different chemicals.